About Bradyrhizobium japonicum and RhizoBase Bradyrhizobium japonicum, a member of rhizobia, has an ability to form root nodules on soybeans (Glycine max). B. japonicum strain USDA110, which was originally isolated from soybean nodule in Florida, USA in 1957, has been widely used for the purpose of molecular genetics, physiology, and ecology, because this strain is superior at symbiotic nitrogen fixation with soybeans as compared with other strains. Bradyrhizobium japonicum (in NCBI TAXONOMY BROWSER) We initiated the genome analysis of B. japonicum strain USDA110 in 2001, and the complete nucleotide sequence of its genome was determined in December 2002. The genome of B. japonicum is a single circular chromosome of 9,105,828 bp long with an average GC content of 64.1%. The chromosome comprises 8317 potential protein-coding genes, one set of rRNA genes and 44 tRNA genes. RhizoBase provides an easy way of accessing the sequcence and all-inclusive annotation data through image maps, keyword searches and the gene category list. This database has been developed and maintained by Yasukazu Nakamura, Takakazu Kaneko and Satoshi Tabata at Kazusa DNA Research Institute. Bradyrhizobium